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SDS Gel Dyes & Buffers

Recipes for running SDS protein gel electrophoresis, SDS sample loading dye, and Coomassie staining protein gels.

5x SDS loading dye

  • 125mM Tris-HCl, pH 6.75
  • 20% (v/v) glycerol
  • 10% (v/v) 2-mercaptoethanol
  • 4% SDS
  • 0.02% bromophenol blue

5x Running buffer (1L)

  • 72 g glycine
  • 15 g Tris-HCl
  • 5 g SDS

Gel Stain

  • 2.5g/L Coomassie Blue
  • 45% (v/v) methanol
  • 45% (v/v) glacial acetic acid

Gel Destain

  • 10% (v/v) acetic acid
  • 10% (v/v) methanol

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