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Tissue culture using flasks vs dishes for cell lines

Flasks and dishes that are cell culture coated are both acceptable consumables to use for cell culture.  Employing sterile techniques allows an experienced scientist to use either item, with pros and cons listed here for each system.

  • Filtered Flasks
    • Narrow opening translates to a lesser chance of contaminant entering the flask
    • Ideal for long term culture due to narrow opening
    • Easy to stack in cramped incubator space
  • Dishes
    • Wide opening allows for easier clonal selection
    • Opening allows for complete removal of washes prior to lysis
    • Greater efficiency for scraping monolayers to recover cells

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