HepG2 Transfection Reagent

Catalog # 2067 - 0.5 mL        $ 195.00 Catalog # 2068 - 1.5 mL        $ 387.00 Transfection reagent for HepG2 cells Transfection Enhancer reagent included Protocols provided for transfection of mRNA, DNA, siRNA and microRNA High transfection efficiency Works well for other liver originated cell lines Works with and without serum Compatible with transfection in any plate format Reproducible transfection Suitable for plasmid DNA/siRNA cotransfection Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

HepG2 cell line

HepG2 was derived from the liver tissue of a 15 year-old caucasian male with differentiated hepatocellular carcinoma. These cells are epithelial and have model chromosome number of 55. The cells secrete a variety of major plasma proteins such as albumin, transferrin, alpha 1-antitrypsin, alpha 2-macroglobulin, and plasminogen (they have been grown successfully in large scale cultivation systems). The cells are responsive to stimulation with human growth hormone. HepG2 cells have also been shown to be resistant to G418. HepG2 cells and its derivatives are used as a model system for studies of liver metabolism and toxicity of xenobiotics, anti genotoxic and cogenotoxic agents, understanding hepatocarcinogenesis, the detection of cytoprotective, and for drug targeting studies. HepG2 cells are also employed in trials with bio-artificial liver devices. Key words: hepatocellular carcinoma, liver, HepG2, epithelial cells, xenobiotics, genotoxic agents, cogeneotoxic agents, human growth harmone, liver cancer, liver carcinoma, carcinoma cells, liver transfection reagent For more information about HepG2 cells visit Wikipedia.

Product Review

Article by Dr.Lakshmanan "A549 Transfection Reagent From Altogen Biosystems": Link

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA, RNA, siRNA, and microRNA enabled by advanced formulation of reagents and peculiar design of protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines. All reagents are functionally tested to be highly reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.