LNCaP Transfection Reagent

Catalog # 1789 - 0.5 mL        $ 197.00 Catalog # 1790 - 1.5 mL        $ 309.00 Transfection reagent for LNCaP cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of siRNA and microRNA High transfection efficiency Work in presence of serum Minimal off-target effects Reproducible results Cost-effective transfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

siRNA Transfection

Gene silencing by RNA Interference (RNAi) is a powerful research tool for studying gene function in mammalian cells. RNAi is a biological phenomenon by which double stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Potent inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds RISC complex and cause degradation of target complementary mRNA molecules in the cell. Therefore, successful, potent RNAi experimentation is dependent upon the highly efficient delivery of the siRNA into cells by transfection of stable and functional siRNA molecules. Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible for transfection of microRNA (miRNA) molecules.

LNCaP cell line

LNCaP cells are commonly used in the field of oncology. LNCaP cells are human prostate adenocarcinoma cells that were derived from the supraclavicular lymph node metastasis of a 50-year-old Caucasian male in 1977. LNCaP cells are adherent epithelial cells growing both in aggregates or as a single cell. The LNCaP cells grow readily in vitro, form clones, and are resistant to human fibroblast interferon. High affinity specific androgen receptors in cytosol and nuclear fractions of cells (in culture and in tumors). Estrogen receptors can be seen in the cytosol. The model is responsive hormonally. The cell line expresses Prostate Specific Antigen. The frequency of the tumor development and the time of tumor appearance are significantly different for males and females. Male mice do develop tumors sooner and at the greater frequency than females. Hormonal manipulations demonstrate that frequency of tumor development correlates with the levels of serum androgen. However, the rate of the tumor growth is not dependent on the gender or hormonal status.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.