Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Transfection Reagent for VERO Cells (African Green Monkey Kidney Cells)
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Two component formulation enhances lipid mediated transfection efficiency
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Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
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Kit includes Transfection Enhancer reagent and recommended transfection protocol
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High transfection efficacy in the presence of serum
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Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
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Reproducible transfection results
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Download in vitro VERO transfection protocol: [PDF]
- Download VERO CRISPR/Cas9 transfection protocol: [PDF]
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Download PowerPoint presentation for VERO cells transfection kit: [PPT]
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Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qPCR.
Product Description:
Optimized transfection kit for high transfection efficiency of VERO cell line. Used in molecular biology to facilitate the introduction of foreign nucleic acids (DNA and RNA) into VERO cells. Compatible with DNA and RNA transfection.
Transfection Protocol and SDS:
Download Altogen Biosystems VEROTransfection Protocol: [PDF]
Download SDS: [PDF]
VERO Cell Line:
The Vero cell line is a continuous cell line that was originally derived from the kidney cells of an African green monkey in the 1960s. Vero cells are widely used in biomedical research and vaccine production because they are easy to grow and can be maintained in culture for long periods of time. Vero cells are particularly useful for the production of viral vaccines, including those for measles, mumps, rubella, and polio. They are also commonly used in virology research to study the replication and pathogenesis of various viruses, including coronaviruses and filoviruses. Continuous mammalian cell lines serve as potent tools for life sciences, aiding in the production of various drugs. The Vero cell line is susceptible to multiple viruses and has widely contributed to the creation of vaccines for human use. The Vero epithelial cell line is taken from kidney cells of an African green monkey (Cercopithecus aethiops). Yasumura and Kawakita isolated these cells in 1962 and named the cell line as an abbreviation of “Verda reno” meaning “green kidney” in Esperanto. Vero cells are aneuploidy, continuous, and can be replicated through numerous cycles of division without becoming senescent. Aneuploidy means having an abnormal number of chromosomes. Unique from mammalian cells, the Vero cell line is interferon-deficient since it does not produce type 1 interferons after being infected by viruses. Vero cells are used for multiple purposes, including screening for the toxins such as Escherichia coli. Also, Vero can serve as a host cell line for growing viruses or eukaryotic parasites (Trypanosomatids.) The Vero cell line is a mammalian cell line commonly used for research in the fields of virology, bacteriology, and toxicology. Also, the Vero cell line is an excellent host for studying a range of areas within the field of biomedical therapeutics. Altogen Biosystems provides highly efficient optimized transfection kits for these cells.
Data:
Figure 1. GAPD mRNA levels were quantified using real-time PCR in the VERO cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
Figure 2. Protein expression of GAPDH in VERO cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into VERO cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 0.5 ml (Catalog #3281)
- 1.5 ml (Catalog #3282)
- 1.5 ml CRISPR (Catalog #2201)
- 8.0 ml (Catalog #7092)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.