Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Transfection Reagent for Hepa 1-6 Cells (Hepatoma Cells, CRL-1830)
- Proprietary cationic lipids formulation
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High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, and plasmid DNA
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Effective and robust cellular delivery
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Kit includes Transfection Enhancer reagent
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A proven reagent for establishing stable cell lines
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Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
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Download Hepa transfection protocol: [PDF]
- Download Hepa CRISPR/Cas9 transfection protocol: [PDF]
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Download PowerPoint presentation for Hepa cells transfection kit: [PPT]
- UPC/GTIN/EAN: 860002089768
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Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Product Description:
Advanced formulation for liver cancer Hepa 1-6 cells. Transfection reagent and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged biomolecules.
Transfection Protocol and SDS:
Download Altogen Biosystems Hepa Transfection Protocol: [PDF]
Download SDS: [PDF]
Hepa 1-6 Cell Line:
Liver cancer is considered the second primary cause of cancer fatalities worldwide that is responsible for more than 30,000 deaths every year in the United States. Approximately 40,000 new cases of liver cancer are diagnosed annually, as stated by the American Cancer Society. Mouse liver cells from the Hepa 1-6 hepatoma cell line are reliable transfection hosts in the study of liver disease. The Hepa 1-6 cell line is initially established from liver cells of the BW7756 mouse (Mus musculus) with hepatoma, also known as liver cancer. The cells are tumorigenic and exhibit epithelial morphology. Hepa 1-6 cells make an excellent transfection host and have been tested negative for mousepox. Also, the Hepa 1-6 cell line is widely used in biomedical research to study mammalian liver tissue functions. Altogen Biosystems provides high-efficiency lipid-based transfection reagent kits for the Hepa1-6 hepatoma cell line.
Hepa1-6 is a mouse liver cancer cell line that is commonly used as a model system for studying liver cancer biology, including tumor growth, invasion, metastasis, and drug resistance. It is one of the most extensively studied mouse liver cancer cell lines and is widely used in cancer research. Hepa1-6 cells are known for their ability to form tumors in vivo when injected into mice, which makes them a valuable tool for studying the interactions between cancer cells and the surrounding microenvironment. Researchers also use Hepa1-6 cells to investigate the molecular mechanisms underlying liver cancer development and progression, and to identify potential therapeutic targets and biomarkers of the disease.
Data:
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into Hepa cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in Hepa 1-6 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into Hepa 1-6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 0.5 ml (Catalog #6820)
- 1.5 ml (Catalog #6821)
- 1.5 ml CRISPR (Catalog #2153)
- 8.0 ml (Catalog #6822)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.