Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Kidney In Vivo Transfection Reagent
siRNA and DNA kidney in vivo delivery reagent for animal research (mouse, rat)
Product Description:
The nanoparticle-based liposomal reagent is a stable complex in serum for 24 hours, demonstrating efficient delivery to kidney tissue and tumors via systemic administration. This reagent is capable of efficiently delivering small RNA (si/sh/miRNA), mRNA, and plasmid DNA with minimal toxicity. Its functionality has been validated in mice and rats and is applicable for plasmid DNA/siRNA co-injection.
Kidney-targeted In Vivo Transfection Reagent
- Nanoparticle-based liposomal reagent
- Nanoparticle/liposome conjugated complexes are stable in serum for 24 hours
- Efficient delivery to the kidney tissue (via systemic administration) and kidney tumors (via intratumoral administration)
- Efficient small RNA (si/sh/miRNA), mRNA, and plasmid DNA delivery
- Minimal toxicity
- Functionally validated in mice and rats
- Applicable for plasmid DNA/siRNA co-injection
- Download Kidney-targeted in vivo transfection protocol: [PDF] [Word]
- Download PowerPoint presentation for Kidney-targeted in vivo transfection kit: [PPT]
- Download safety data sheet: [PDF]
- Developed and manufactured by Altogen Biosystems
Modes of administration:
- Systemic intravenous (i.v.) injection
- Direct intratumoral (i.t.) injection
- Intraperitoneal (i.p.) injection
DATA
Figure 1. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).
Figure 2. Intratumoral administration (i.t.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (kidney cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).
Figure 3. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.
Kidney Transfection Reagent citations:
- The Journal of Pathology. 2015 235(5):731–744. BDNF repairs podocyte damage by microRNA-mediated … Min Li et al [PDF]
- Nature Communications. 2016. 7:12076. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Badal et al [PDF]
- Journal of the American Society of Nephrology. 2016 Nov;27(11):3308-3319. Synaptopodin Limits TRPC6 Podocyte Surface Expression and Attenuates Proteinuria. Yu H et al [PDF]
- British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
- Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
- J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]
Altogen Labs Contract Research Services:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Kidney-targeted Transfection Kit
This nanoparticle-based liposomal reagent is a stable complex in serum for 24 hours, demonstrating efficient delivery to kidney tissue and tumors via systemic administration. Effective delivery of small RNA (si/sh/miRNA), mRNA, and plasmid DNA were functionally validated in laboratory mice and rats. Nanoparticles and liposomes ensure the stability, delivery efficiency, and minimal toxicity. The development of this nanoparticle-based liposomal reagent represents a significant advancement in targeted drug delivery systems. By achieving stable and efficient delivery of genetic material such as siRNA, shRNA, miRNA, mRNA, and plasmid DNA, this technology has the potential to benefit gene therapy and precision medicine. The ability to specifically target kidney tissue and tumors minimizes off-target effects and reduces systemic toxicity, addressing a critical challenge in current therapeutic strategies. The successful functional validation in animal models paves the way for potential clinical applications, offering new hope for treating kidney-related diseases and cancers with higher efficacy and safety profiles. This innovation not only enhances our understanding of nanoparticle and liposome behavior in biological systems but also opens new avenues for the development of personalized medicine approaches.
Volume Options:
- 0.5 ml – 10 injections (Catalog #5070)
- 1.5 ml – 30 injections (Catalog #5071)
- 8.0 ml – 160 injections (Catalog #5072)
- 25 ml – 50 rat injections or 500 mouse injections (Catalog #5073)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.